豚鼠醛化細(xì)胞懸液（多種濃度）

【產(chǎn)品名稱】 ：1%豚鼠醛化細(xì)胞懸液

【貨號】：FXQ364-01

【產(chǎn)品規(guī)格】 ：100ml                            

【儲存條件】 ：2～8℃                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               

【有效期】 ：三個月 

醛化紅細(xì)胞特點:
1.醛化紅細(xì)胞鏡檢時一般呈兩面凸起的園盤狀，散在，不聚集成團(tuán)。
2.紅細(xì)胞醛化后沉積細(xì)胞體 積約增加20%，紅細(xì)胞固有形態(tài)無變化，呈棕色，在有Na+存在時顏色略紅。
3.醛化細(xì)胞具有較強(qiáng)的抗破壞能力，反復(fù)凍融，加熱至60℃，蒸餾水中低滲均不破壞，凍干后重新以水懸浮并無明 顯變化，pH<3.0時，可自然凝集，但pH達(dá)1.8時也不溶血;pH≥10.2可有中度溶血。醛化細(xì)胞懸液4℃可存放3個月以上，但凍結(jié)后重新融化則凝集成團(tuán)。 

戊二醛化紅細(xì)胞制備法:
沉積紅細(xì)胞用10~20倍0.11MpH7.2 PB洗4~6，再次配成10%懸液，取此懸液 (預(yù)先冷至4℃) 緩慢地加入到等量 1%的戊二醛(25%戊二醛用0.11MpH7.2PB稀釋 之)中，并繼續(xù)搖動30~60分鐘，然后用蒸餾水洗五次。最后用重蒸餾水 配成20%的懸液，加0.1%NaN3防腐，4℃冰箱保存?zhèn)溆谩?/font>
丙酮醛一一甲醛化紅細(xì)胞制備:
(1)沉積紅細(xì)胞以10~20倍體積 0.11MpH7.2PB洗滌4~6次，然后用該PB配成8%紅細(xì)胞懸液。
(2)8%紅細(xì)胞懸液中,緩慢加入等量3%丙酮醛、3%甲醛PB液(取丙酮醛15ml、甲醛8ml、0.11MpH7.2PB60ml,用10%Na-OH調(diào)正pH至7.2再加同樣PB至100mI)于20℃左右緩慢攪拌17小時，至?xí)r用0.11MpH7.2PB洗三次，并用同樣PB制成20%紅細(xì)胞懸液，加入0.1%NaN3防腐，4℃冰箱保存?zhèn)溆谩?/font>

注意事項：
1.在加醛類前要盡量將紅細(xì)胞洗凈;
2.開始加入甲醛濃度要淡，以后逐漸提高甲醛濃度，否則紅細(xì)胞容易變形;
3.作適當(dāng)?shù)膿u動，使甲醛與紅細(xì)胞充分接觸。

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